Table of Contents

Part I: the Analytical Problem

Investigation 1: Properties of Danshen's Constituent Compounds

Part II: Separating and Analyzing Mixtures Using HPLC with UV Detection

Investigations 2–3: Reverse-Phase HPLC and Order of Elution of Solutes
Investigations 4–6: UV Detection and Beer's Law

Part III: Extracting Analytes from Samples

Investigations 7–9: Solvent Extraction of Danshen

Part IV: Optimizing the Solvent, Temperature, and Microwave Power

Investigation 10: One-Factor-at-a-Time Experimental Optimizations
Investigations 11–13: Optimizing the Solvent (Part A)
Investigation 14: Optimizing the Solvent (Part B)
Investigation 15: Optimizing the Temperature (Part A)
Investigation 16: Optimizing the Temperature (Part B)
Investigation 17: Optimizing the Microwave's Power (Part A)
Investigation 18: Optimizing the Microwave's Power (Part B)

Part V: Optimizing the Solid-to-Solution Ratio and the Extraction Time

Investigations 19–21: Optimizing Two Factors at Once Using a Central-Composite Design
Investigations 22–23: Modeling the Effect of Solid-to-Solution Ratio and Extraction Time on Extraction Yield of Danshensu
Investigations 24–25: Modeling the Effect of Solid-to-Solution Ratio and Extraction Time on Extraction Yields of Other Analytes

Part VI: Findng the Global Optimum Across all Analytes

Investigation 26: Individual Desirability Functions
Investigations 27–29: The Global Desirability Function and the Total Extraction of Danshen

Part VII: Verifying the Analytical Method's Accuracy

Investigation 30: Extraction Yield for Danshensu
Investigation 31: Extraction Yields for Other Analytes
Investigation 32: Comparing Results to Other Analytical Methods
Investigation 33: Spike-Recovery Studies

Part VIII: Applying the Analytical Method

Investigation 34: Analysis of Wild and Cultivated Samples of Danshen

Part IX: Summary of case study

Closing Thoughts

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